Background: The medical laboratory has a fundamental role in diagnosing diseases, following up and correcting treatment at the right time. The quality of laboratory result was affected by multiple factors through the stages of work. Objective: The study aimed to identify impact factors of poor quality management of clinical laboratories’ performance in Wad Medani city, Sudan. Methods: A descriptive cross-sectional study was followed; enrolled medical laboratories were seven governmental hospital laboratories and nine private laboratories. Medical laboratory design assessment data, personnel management and sampling, and general concepts of quality control were collected through the questionnaire. Results: The overall observed rates for proper design and structure, personal management, sample management, and quality control requirements in medical laboratories were 22.5%, 39.25%, 23.45%, and 30.50%, respectively. Conclusion: Several impact factors in design, person management, sampling and overall quality led to poor performance in medical laboratories in Wad Medany city.
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